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. 2020 Oct 2;10:16378. doi: 10.1038/s41598-020-71849-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antiviral activity of PCME against DENV infection. (A) Cytotoxicity of PCME on Vero cells as analyzed by XTT cell viability assay. DMSO (1%) served as negative control treatment. (B) Immunostained plaque assay-based antiviral dose response analysis of PCME treatments against DENV infection (100 PFU/well). DMSO (0.06%) served as negative control and data is expressed as percent (%) DENV infectivity. (C) The 50% cytotoxic concentration (CC₅₀), 50% effective concentration (EC₅₀), and selectivity index (SI = CC₅₀/EC₅₀) values of PCME against DENV infection. Data shown are means ± SEM (*P < 0.05) from 3 independent experiments.