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. 2020 Oct 2;10:16378. doi: 10.1038/s41598-020-71849-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PCME targets the early phase of DENV infection. (A) Schematics of the time-of-drug-addition analysis. (B) Effect of PCME pretreatment on cells prior to DENV infection. (C) Coaddition treatment analysis of PCME during DENV infection of cells. (D) Postinfection treatment effect of PCME on DENV-infected cells. Results were obtained using immunostained plaque assay in Vero cells following 6 days of incubation. PCME = 30 μg/ml; DENV = 100 PFU/well; DMSO = 0.06%. Data shown are means ± SEM (*P < 0.05) from 3 independent experiments.