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. 2020 Oct 2;10:16378. doi: 10.1038/s41598-020-71849-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PCME inactivates free DENV particles and blocks viral attachment and entry/fusion steps. (A) Schematics of the synchronized infection analysis on DENV early viral entry. (B) Treatment effect of PCME on cell-free DENV particles. (C) Effect of PCME on DENV attachment. (D) Influence of PCME on DENV entry/fusion. All data were obtained by immunostained plaque assay in Vero cells at the end-point (6 days) of the experiments. PCME = 30 μg/ml; final DENV concentration = 100 PFU/well; DMSO = 0.06%. Data shown are means ± SEM (*P < 0.05) from 3 independent experiments.