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. 2020 Oct 2;10:16378. doi: 10.1038/s41598-020-71849-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of PCME-mediated inhibition on DENV viral entry using flow cytometry. (A) Effect of PCME treatment on DENV viral infection of host cells. Cells were infected with DENV in the presence of the test drug and subjected to flow cytometry detection of intracellular DENV E protein expression. (B) Analysis of mean fluorescence intensity from (A). Data shown are means ± SEM (*P < 0.05) or representative histogram from 3 independent experiments.