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. 2020 Oct 2;10:16378. doi: 10.1038/s41598-020-71849-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antiviral activity of PCME treatment is not mediated through influence on DENV replication, translation, or virion release. (A) Treatment effect of PCME on DENV intracellular replication. DENV genomic RNA was extracted from DENV-infected Vero cells following 3 days of drug treatment and quantitated using qRT-PCR; DENV MOI = 0.1. (B) Luciferase reporter analysis reflecting viral replication/translation from subgenomic DENV replicon Huh7.D2-FLuc-SGR-Neo cells treated with PCME for 3 days. Data are presented in log₁₀ of relative light units. (C) Effect of PCME on DENV proteins expression from Western blotting analysis of lysates obtained from DENV-infected Vero cells following 3 days of drug treatment. DENV MOI = 0.1; β-actin served as loading control. (D) Effect of PCME on DENV release via Western blotting analysis of supernatants from DENV-infected cells after 3 days of drug treatment. PCME = 30 μg/ml; Ribavirin = 200 μM; DMSO = 0.06%. Data shown are means ± SEM (*P < 0.05) or representative immunoblots from 3 independent experiments.