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. 2020 Oct 2;10:16378. doi: 10.1038/s41598-020-71849-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PCME impedes DENV infection of various cell types. Analysis of DENV infectivity in (A) Vero (DENV MOI = 0.1), (B) Huh-7 (DENV MOI = 1), (C) C3/C6 (DENV MOI = 0.1), (D) U937 (DENV MOI = 1), and (E) K562 (DENV MOI = 1) cells in the presence or absence of PCME treatment. For U937 and K562 cells, subneutralizing concentration (1: 100) of the 4G2 antibody was added at the time of infection to enhance the DENV infection via antibody enhancement. Following washes, the cells were incubated for 3 days prior to detection of DENV E protein by flow cytometry. Data shown are means ± SEM (*P < 0.05) from 3 independent experiments.