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. 2020 Oct 2;10:16378. doi: 10.1038/s41598-020-71849-3

Figure 9.

Figure 9

PCME inhibits the entry of HCV and ZIKV but not WNV. (A) Huh-7 cells seeded in 96-well plates were challenged with reporter-tagged HCVcc (MOI = 0.01) in the presence of 10 μg/ml PCME for 1.5 h. Cells were then washed with PBS and incubated in basal media for 72 h, before evaluating the Gaussia luciferase reporter activity. (B) Vero cells were seeded overnight in 6-well plates and infected with ZIKVic (MOI = 0.1) in the presence of 30 μg/ml of PCME for 2 h. The cells were then washed and incubated in growth media for 72 h, before being subjected to the flow cytometry-based infection analysis. (C) Vero cells seeded in 96-well plates were infected with WNV.RVPs (MOI = 0.1) for 2 h in the presence of 30 μg/ml PCME. The cells were subsequently washed and incubated in basal media for 72 h, before assessing the Renilla luciferase activity. Data shown are means ± SEM (*P < 0.05) from 3 independent experiments.