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. 2020 Oct 2;10:16418. doi: 10.1038/s41598-020-73414-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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2D microarrays: (a, f) Optical images; (b, g) Green autofluorescence; Representative fluorescence microscope images of fibroblasts seeded on 2D microarrays: (c, h) cytoskeleton (red) visualized by staining with F-actin; (d, i) nuclei (blue) visualized by staining with Hoechst; (e, j) overlapped fluorescence images of 2D microarrays, cells cytoskeleton and nuclei; (k, m) 3D top and (l, n) 3D tilted views of cells nuclei (blue), cytoskeleton (red) and centers of MNPs and agglomerations of MNPs (yellow), imaged by enhanced dark field microscopy. The samples were imaged in the presence (a–e, k, l) and absence (f–j, m, n) of SMF. All cells were imaged after 1 day of cell culture.