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. 2020 Oct 2;11(10):822. doi: 10.1038/s41419-020-03030-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a qRT-PCR was used to analyze HuR mRNA levels in HuH7 and HepG2 cells following siRNA-mediated AFP knockdown. b Western blotting showed the effects of AFP overexpression or knockdown on total and cytoplasmic HuR protein levels. Left panel, HLE cells were stably transfected with an AFP expression plasmid. Right panel, AFP expression was knocked down in HuH7 and HepG2 cells by siRNA-mediated interference. c The AFP-altered cellular HuR distribution was detected by immunofluorescence staining and confocal microscopy imaging. Up, AFP was overexpressed in AFP-negative HLE cells; bottom, AFP was silenced in HuH7 cells. Scale bar, 20 μm. d Coimmunoprecipitation was used to analyze the HuR and AFP interaction in HCC cells. HLE cells were stably transfected with the AFP expression plasmid. e The colocalization of AFP and HuR in the HuH7 or HepG2 cell cytoplasm was captured by confocal microscopy. Scale bar, 20 μm.