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. 2020 Oct 2;11:4950. doi: 10.1038/s41467-020-18750-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Intestinal wall perfusion was measured using Doppler ultrasound and calculated as average flow velocity (mm/s) of multiple abdominal regions. b Intestinal wall perfusion measured daily from P5 to P9 showed reduced perfusion in NEC pups which was improved following conditioning with Stage 1 or 2 RIC (n = 6 per group; minimum of 3 readings of different abdominal quadrants were obtained per pup; **p < 0.01; ***p < 0.001). c Intestinal wall perfusion measured on P9 in NEC pups showed reduced perfusion compared to breastfed (BF) control, which was preserved in NEC pups receiving Stage 1 or 2 RIC (n = 6 per group; minimum of 2 readings of different abdominal quadrants were obtained per pup; **p < 0.01; ***p < 0.001). d Representative images of arterial waves obtained with the Doppler ultrasound in P9 pups from listed groups. Data were compared using two-sided one-way ANOVA with post hoc Turkey test and data are presented as mean ± SEM. Source data are provided as a Source Data file.