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. 2020 Oct 2;16:371. doi: 10.1186/s12917-020-02595-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Time-dependent manner of baicalin inhibition on MDV replication in CEF. Total cellular RNA was extracted from day 1 to day 5 p.i., and the expression levels of Meq gene (a) and gB gene (b) were detected by qRT-PCR. c The results of plaque count of the MDV RB-1B strain infected CEF treated with 0 and 20 μg/mL of baicalin at different time points. d Direct observation of viral plaque formation dynamics by indirect immunofluorescence assays from day 1 to day 5 post virus infection. Data were expressed as mean ± SD is for A, B and C only from three independent experiments and analyzed by Student’s t-tests (**p < 0.01, *** p < 0.001)