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. 2020 Oct 2;19:187. doi: 10.1186/s12934-020-01442-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 11 — Assay of the substrate conversion catalyzed by KshA226 and KshA395 in combination with KshB122. a–c SDS-PAGE evaluation of purified KshA226, KshA395 and KshB122 fused with MBP. M, Molecular weight markers, which were run on the same gel. In a, sample 2 was run on a different gel simultaneously with the same electrophoresis conditions. In c, sample 2 was run on a different gel simultaneously. d TLC assay of the conversion of ADD to HSA catalyzed by KshA226 and KshA395, respectively, in combination with KshB122. Lanes 1 and 5, ADD standard; lanes 4 and 8, HSA standard; lanes 2 and 6, the reaction mixtures catalyzed by KshA226/KshB122 and KshA395/KshB122, respectively, for 5 h; and lanes 3 and 7, the reaction mixtures catalyzed by KshA226/KshB122 and KshA395/KshB122, respectively, for 20 h. e TLC evaluation of the conversion of 4-AD to 9OH-AD catalyzed by KshA226 or KshA395 in combination with KshB122 within 20 h. Lane 1, 4-AD standard; lane 4, 9OH-AD standard; lanes 2 and 3, KshA226/KshB122 and KshA395/KshB122, respectively, for 5 h; f HPLC assay of the conversion of 4-AD to 9OH-AD catalyzed by KshA226 or KshA395 in combination with KshB122 for 40 h. The peaks for 4-AD and 9OH-AD were marked. g Enzyme-catalyzed conversion of 4-AD to 9OH-AD by KshA395 in combination with KshB122 for 40 h. Error bars indicate standard errors of the means of three repeat experiments