Figure 1.

Experimental approach: a) Mice bearing bilateral subcutaneous tumors (volume of ~250 mm³ each) received a systemic bolus (100 μg) of fluorescently labeled antibodies (anti-CD73-F) and ultrasound targeted microbubble cavitation (UTMC) therapy on the right side during the infusion of microbubbles (Definity) via the tail vein. UTMC was guided by contrast enhanced ultrasound imaging (CEUS) allowing monitoring of the spatially targeted therapy and microbubble replenishment. Mice were then imaged for biofluorescence and sacrificed at 0 h (<10min), 4 h and 24 h post UTMC for confocal microscopy.