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Fluorescently labeled mAb (anti-CD73-F) binding to 4T1 cells: (a) 4T1 cells were either exposed to fluorescently labeled antibodies (anti-CD73-F), or successively to anti-CD73 and anti-CD73-F in a competitive binding assay; (b) This competing assay with a prior native mAb challenge reduced the fluorescence/binding of anti-CD73-F, without changes in cell confluence (N=3); (c) Confocal microscopy supports that CD73 was expressed mostly on the membrane of 4T1 cells. Scalebar = 25μm.
