Table 1.

Summary of sampling locations, sponge features classified according to (i) host nutritional mode (presence or absence of photosymbionts); (ii) microbial symbiont strategy (high or low microbial abundance) and sequencing statistics. N50 values for each dataset were calculated based on evaluation of unfiltered contigs

Host species/replicate	Sampling site1	Hosts photo-symbionts	Microbial abundance	Raw reads* (#)	Contig N50	Contigs (#)	Longest contig (bp)	Predicted genes (#)	Taxonomically assigned genes (%)
Callyspongia sp. 1	GBR	Yes	LMA	11.06	899	23,339	58,159	63,281	23.5
Callyspongia sp. 2	GBR	Yes	LMA	9.36	932	20,309	172,832	56,308	23.6
Callyspongia sp. 3	GBR	Yes	LMA	8.97	810	21,236	69,654	56,363	24.0
C. foliascens 1	GBR	Yes	HMA	4.92	675	33,078	24,096	57,288	13.2
C. foliascens 2	GBR	Yes	HMA	1.78	698	14,225	46,996	25,768	11.0
C. foliascens 3	GBR	Yes	HMA	15.28	624	45,237	23,964	76,516	13.0
C. schulzei 1	GBR	No	HMA	7.95	467	9621	80,905	17,001	14.8
C. schulzei 2	GBR	No	HMA	8.23	571	12,170	81,025	26,002	17.5
C. schulzei 3	GBR	No	HMA	19.59	660	50,864	80,778	95,389	14.4
C. marshae 1	GBR	Yes	HMA	1.53	434	4549	9804	7975	16.2
C. marshae 2	GBR	Yes	HMA	3.75	398	5419	7498	6585	16.6
C. marshae 3	GBR	Yes	HMA	3.12	405	6751	12,455	10,427	16.1
E. isaaci 1	GBR	NA	NA	4.20	566	11,349	31,106	21,794	22.5
E. isaaci 2	GBR	NA	NA	4.50	510	13,898	49,604	28,567	23.3
E. isaaci 3	GBR	NA	NA	1.26	694	4752	42,237	11,022	21.8
I. basta 1	GBR	No	LMA	6.58	504	11,600	60,819	24,285	19.3
I. basta 2	GBR	No	LMA	4.63	495	12,591	39,149	23,850	22.2
I. basta 3	GBR	No	LMA	4.83	552	14,561	36,340	28,801	20.8
L. herbacea 1	GBR	Yes	LMA	7.96	457	11,693	43,955	21,907	20.2
L. herbacea 2	GBR	Yes	LMA	14.88	594	9661	14,898	18,144	19.2
L. herbacea 3	GBR	Yes	LMA	3.02	504	16,931	43,769	30,283	20.2
P. candelabra 1	GBR	NA	NA	12.20	693	35,688	49,806	32,910	15.0
P. candelabra2	GBR	NA	NA	7.74	557	27,010	58,681	48,732	14.4
P. candelabra 3	GBR	NA	NA	5.87	958	13,373	120,555	29,643	18.2
S. carteri 1	GBR	No	LMA	6.34	346	4063	10,851	5709	17.6
S. carteri 2	GBR	No	LMA	4.78	384	7833	16,548	13,143	21.9
A. ochracea 3	Red Sea	Yes	LMA	3.38	720	7706	26,892	18,040	25.6
A. ochracea1	Red Sea	Yes	LMA	14.14	717	29,033	61,876	71,543	23.2
A. ochracea 2	Red Sea	Yes	LMA	2.79	694	7692	20,756	10,981	21.1
C. foliascens 1	Red Sea	Yes	HMA	2.30	1671	2571	35,948	7096	20.7
C. foliascens 2	Red Sea	Yes	HMA	1.64	1135	3913	49,410	10,708	19.7
C. foliascens 3	Red Sea	Yes	HMA	1.82	1280	2787	38,097	7284	21.4
C. cyathophora 1	Red Sea	Yes	LMA	3.19	601	2757	19,321	8636	25.6
C. cyathophora 2	Red Sea	Yes	LMA	3.58	618	2613	39,999	5753	24.9
C. cyathophora 3	Red Sea	Yes	LMA	1.84	614	3977	44,834	7951	21.5
H. erectus 1	Red Sea	No	HMA	18.37	921	6400	7865	17,674	21.8
H. erectus 2	Red Sea	No	HMA	6.04	878	9540	47,148	25,550	23.9
Mycale sp. 1	Red Sea	NA	NA	5.81	949	5396	39,546	13,458	16.1
Mycale sp.2	Red Sea	NA	NA	5.74	705	9562	54,476	22,104	22.7
Mycale sp. 3	Red Sea	NA	NA	5.06	682	6123	22,927	13,601	20.2
N. rowi 1	Red Sea	NA	NA	2.40	668	6058	25,175	13,766	27.5
N. rowi 2	Red Sea	NA	NA	7.35	922	10,017	52,589	27,503	24.0
X. testudinaria 1	Red Sea	Yes	HMA	6.13	881	7533	103,486	20,344	16.2
X. testudinaria 2	Red Sea	Yes	HMA	7.40	729	9639	74,732	23,194	17.1
Sea water—GBR	GBR	NA	NA	21.64	406	11,008	20,625	19,950	22.0
Sea water—RS	Red Sea	NA	NA	9.51	523	10,568	52,589	36,395	35.1

¹GBR sponges were collected from Orpheus Island, Queensland, Australia (18° 35′ 34″ S, 146° 28′ 53″ E) and Red Sea sponges were collected in Al Fahal, Saudi Arabia (22° 13′ 95″ N, 39° 01′ 81″ E)

*Raw read values are presented as million of reads. Taxonomic assignments are based on pairwise sequence similarity to the Viral RefSeq database using MEGAN LCA parameters [30]