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. 2020 Sep 11;10(24):11144–11158. doi: 10.7150/thno.42578

Figure 3.

Figure 3

Cyclin K participates in G2/M checkpoint control and the DNA damage response. (A) Cyclin K silencing abrogated IR-induced G2/M arrest. Cells were transfected with indicated siRNAs for 24 h and then exposed to IR. After another 24 h, cells were harvested and analyzed by flow cytometry. **P < 0.01. ***P < 0.001 (n = 3). (B) Cyclin K depletion abolished the G2/M checkpoint. Cells transfected with siRNA and then exposed to IR were treated with nocodazole (100 ng/mL) for 22 h before being harvested. The samples were analyzed by flow cytometry. The percentage of cells positive for phospho-histone H3 (pH3) is indicated (n = 3). (C) Cells were transfected with siRNA and then exposed to IR. After 4 h, cells were harvested and seeded to comet slides, then lysed for electrophoresis. ***P < 0.001 (n = 3). Scale bar, 10 µm. (D) Left panel: Cells transfected with indicated siRNAs were irradiated and then harvested after 4 h or 24 h. Representative immunostaining images are shown. Right panel: Quantification results of γ-H2AX foci are also shown. Scale bar, 10 µm. **P < 0.01. ***P < 0.001 (n = 3).