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. 2020 Sep 11;10(24):11159–11177. doi: 10.7150/thno.44389

Figure 2.

Figure 2

Elevated Rbm24 expression during skeletal muscle regeneration. (A) Rbm24 expression was upregulated in the TA muscle of mdx mice compared with the WT mice of the same age. The 4-week-old mdx mice were used for the pathological muscle regeneration model. For each group, 2 samples were used as representatives. GAPDH was used as a loading control. (B) Histogram showing the expression levels of Rbm24 in (A), Rbm24 was normalized to GAPDH. Data are presented as mean ± SEM, n = 4, ***P < 0.001, unpaired t-test. (C) Schematic diagram depicting CTX-induced injury and specimen collection. TA muscle of 2-month-old WT mice was injured by CTX injection and samples were collected at the indicated time points. (D) Immunostaining analysis of Rbm24 expression in uninjured and injured TA muscle. TA muscle of 2-month-old WT mice was used for analysis of the localization of Rbm24 before and after injury. Muscle injury was induced by injection of CTX. After 5 D.P.I, TA muscle was collected for analysis. Scale bars, 20 µm. (E) Expression profiles of Rbm24 and regeneration markers (Pax7, Myod1 and Myog) during skeletal muscle regeneration. Data are presented as mean ± SEM, n = 3. (F) Western blot analysis of Rbm24 expression during muscle regeneration. GAPDH was used as the loading control. Abbreviations: CTX: cardiotoxin; D.P.I: days post injury; mdx: X-linked muscular dystrophy; TA muscle: tibialis anterior muscle; UI: uninjured; WT: wild type.