Figure 6.

Transcriptome remodeling in the Rbm24 knockout mice. (A) Bar chart showing the number of DEGs and genes that underwent AS in Rbm24 knockout mice. (B) Pie chart showing the distribution of different types of AS events. (C) GO analysis of genes that were alternatively spliced in response to loss of Rbm24. Genes that were involved in cell differentiation, muscle cell development and muscle hypertrophy were significantly enriched. (D) A table showing the genes that were alternatively spliced by Rbm24 and involved in cell morphogenesis during differentiation and muscle cell development. (E) RT-PCR analysis to validate the AS of genes mentioned in (D). Total RNAs were extracted from intact TA muscle 3 days after tamoxifen treatment. The primers used for RT-PCR are indicated by the arrows. Hprt was used as the loading control. White box, flanking constitutive exons; gray and black box, alternative exons. Abbreviations: AS: alternative splicing; A5SS, A3SS: alternative 5' and 3' splice sites; DEG: differential expression genes; GO: Gene Ontology; MXE: mutually exclusive exons; RI: retained exons; RT-PCR: reverse transcription-polymerase chain reaction; SE: skipped exons.