Figure 7.

Identify altered AS events in Rbm24 knockout mice which are indispensable for myogenesis and muscle regeneration. (A) A network of AS events that are essential for muscle development and homeostasis. (B-D) RT-PCR analysis of AS events involved in (B) muscle hypertrophy, (C) myogenesis and muscle regeneration and (D) myopathy. The RNA samples were collected from TA muscle of 2-month-old mice 3 days after tamoxifen treatment. The arrowheads indicate the primer location and the dark or gray boxes represent the alternative exons. Hprt was used as the loading control. (E) A table showing the genes with isoform-specific functions in myogenesis or muscle regeneration. (F) RT-PCR analysis of AS of genes listed in (E) during regeneration. Naca is alternatively spliced to generate the skNac (muscle specific isoform) and αNac (ubiquitously expressed isoform). Total RNAs were extracted from injured TA muscle of 2-month-old mice at different time points following injury. The primers used for RT-PCR are indicated by the arrows. White box, flanking constitutive exons; gray and black box, alternative exons. Abbreviations: AS: alternative splicing; D.P.I: days post injury; RT-PCR: reverse transcription-polymerase chain reaction; TA muscle: tibialis anterior muscle.