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. 2020 Sep 2;10(24):10993–11012. doi: 10.7150/thno.43094

Figure 1.

Figure 1

The schematic diagram for Suntag-mediated single molecule RNA snapshot method (CRISPR-Sunspot). For CRISPR-Sunspot, the imaging components, dCas9-24 × GCN_v4 and scFv-sfGFP proteins with NLSs were expressed under the Tet-on system for inducible control. With the guide of sgRNA and PAMmer targeting mRNA, fluorescent proteins could be recruited to single-molecule mRNA for signal amplification. In combination with CRISPR activation (CRISPRa), CRISPR-Sunspot could selectively activate transcription and illuminate the produced target mRNA molecules, simultaneously.