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. 2020 Aug 29;10(24):10874–10891. doi: 10.7150/thno.47037

Figure 5.

Figure 5

ERRα acts as an indispensable mediator of estrogen/ERα signaling in transcriptional regulation of VLDL-related genes. (A) Immunoblot analysis of ERα, ERRα, ApoB, MTP and PLA2G12B expression in female Flox and ERRαLKO hepatocytes in the presence of ERα transfection (left panel). Densitometry analysis of the western blotting data normalized to the intensity of Histone H3 or β-actin (right panel). (B) Top, sequence logos for ERRE (left) and ERE (right). Bottom, schematic representations of wild-type mouse Esrra, Apob, Mttp and Pla2g12b promoters and mutation constructs. (C-D) Chromatin immunoprecipitation analysis to examine occupancy of ERRα (C) and ERα (D) at the indicated genes in liver tissue from female wild-type mice fasted overnight were determined by qRT-PCR. (E-G) Luciferase reporter assays showing effect of ERRα w/o its co-activator PGC-1α on promoter serial constructs in HEK293T cells. Each ratio was normalized to the control (pcDNA4.0 vector) (n = 5). Data presented as means ± SD. *P < 0.05, ** P < 0.01, ***P < 0.001, two-way ANOVA followed by Bonferroni post analysis. Abbreviations: ERRE, ERRα response elements; ERE, estrogen receptor response elements; Mut, mutation.