Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 14;10(24):11302–11323. doi: 10.7150/thno.47746

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

Leptin activation was dependent on the AMPKα/STAT3 pathway. (A) Relative mRNA expression of the BAT markers UCP-1, PGC1α, PRDM-16, DIO-2, and CD36 in differentiated C3H10T1/2 cells treated with vehicle, leptin or PNS. (B) Western blot analyses of PRDM-16, PGC1α, leptin, and phosphorylated STAT3 and AMPKα protein levels in differentiated C3H10T1/2 cells. β-actin was used as a loading control. (C) The efficiency of the siRNA-mediated depletion of AMPKα and STAT3 in beige cells treated with leptin was determined by immunoblotting. (D) Representative fluorescence images showing the expression of UCP-1 (red) and the status of lipid droplets stained with BODIPY 493/503 (green) in the siRNA-treated cells after induction. Scale bar: 20 µm.