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. 2020 Aug 29;10(24):10925–10939. doi: 10.7150/thno.49425

Figure 3.

Figure 3

BASP1 enhances EGFR signaling and EGFR protein expression to promote lung cancer progression. (A) Lysates of BASP1-knockdown A549 and Bm7 lung cancer cells were subjected to immunoblotting with the indicated antibodies. (B and C) Western blot of EGFR signaling pathway proteins in A549 (B) and CL1-0 (C) lung cancer cells overexpressing BASP1-GFP. (D) Western blot of BASP1 and EGFR in lung cancer cell lines (A549, Bm7, and H2981) with IPTG-inducible shBASP1 expression. (E) Western blot of BASP1 and EGFR in HEK293T cells cotransfected with BASP1 and EGFR expression plasmids. The amount of transfected plasmids is shown above the blots. (F and G) BASP1 knockdown-mediated inhibition of cell proliferation was rescued by EGFR overexpression. IPTG-induced BASP1 knockdown by shBASP1 in A549 (F) and H2981 (G) cells. Vector, control cells without IPTG induction. EGFR plasmids were transfected into lung cancer cells 2 days after IPTG induction and subjected to MTT assays to determine cell proliferation. (H and I) Intracellular calcium concentrations of control and IPTG-inducible BASP1 knockdown in A549 (H) and H2981 (I) lung cancer cells. Cells were serum starved for 4 hours and then treated with 50 ng/mL EGF. Data represent the mean ± SD. *P < 0.05, Student's t-test.