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. 2020 Sep 2;10(24):11063–11079. doi: 10.7150/thno.45674

Figure 3.

Figure 3

CSRP2 depletion promotes the progression of CRC cells in vitro and in vivo. (A) Western blot analysed confirmed the successful construction of stable CSRP2 depletion in SW480 and HCT15 cells. (B) qPCR analysed confirmed the successful construction of stable CSRP2-depletion in CRC cells. (C) CCK8 assay assessed the proliferation ability of CSRP2-depleted CRC cells. (D) Colony formation assay assessed the proliferation ability of CSRP2-depleted CRC cells. (E) Transwell invasion assay assessed invasive ability of CSRP2-depleted CRC cells. (F) Wound healing assay showed migration capability of CSRP2-depleted CRC cells. (G) The determination of subcutaneous tumor of nude mice respectively constructed by CSRP2-depleted CRC cells and control cells. (H) H&E, Ki-67 and E-cadherin staining of subcutaneous tumor. (I) Representative photographs of liver metastasis of CSRP2-depleted SW480 cells and control cells on 30 and 60 days post-inoculation by in vivo fluorescence imaging (left). Summary results of nude mice presenting with liver metastasis between CSRP2-depleted SW480 group and control group (right). (J) Dissection of intestinal and liver in in situ implant CRC nude mice. (K) The intestinal and liver tissues were stained with H&E and observed under the light microscope. Data are shown as mean ± SEM.