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. 2020 Sep 2;10(24):11063–11079. doi: 10.7150/thno.45674

Figure 4.

Figure 4

CSRP2 inhibits EMT and modulates p130Cas phosphorylation and Rac1 activation in CRC cells. (A) Western blot was performed to detect the EMT marker proteins expression alteration of CSRP2-overexpressed or -depleted cells compared to control cells. (B) IF staining analyzed the EMT marker proteins expression alteration of CSRP2-overexpressed or -depleted cells compared to control cells. (C) Interaction between CSRP2 and p130Cas was confirmed by co-immunoprecipitation assay. (D) IF staining assay with Z-stack was used to confirme the interaction between CSRP2 and p130Cas. Using co-location scatter diagram and PCC to analyze co-location (PCC: Pearson correlation coefficient). (E) Western blot was performed to detect the protein expression changes of p130Cas and phosphorylated p130Cas (p-p130Cas) between CSRP2-overexpressed or -depleted cells and control cells. (F) Immunohistochemical detection of the correlation between CSRP2 and p-p130Cas in serial sections of the same CRC tissues and adjacent normal mucosa. (G) The chart is the statistics of correlation coefficient of figure F. (H) Protein expression alterations of Rac1, RhoA, Cdc42, and their active form, were detected by western blot in CSRP2-overexpressed or-depleted cells and control cells.