Skip to main content
. 2020 Sep 2;10(24):11063–11079. doi: 10.7150/thno.45674

Figure 6.

Figure 6

CSRP2 regulates Hippo, ERK, and PAK signaling pathways through p130cas/Rac1 mediation to inhibit CRC cell invasion and EMT. (A) WB was performed to deteced the expression of total p130Cas and p-p130Cas in CSRP2-depleted CRC cells and control cells treated with wild-type and mutant p130Cas. (B) Rac1 activation was detected by WB. (C) WB was performed to detect the activation of LATS1, YAP in Hippo signal pathways and the activation of ERK in ERK signal pathways, and the protein expression changes of EMT markers. (D) The expression of E-cadherin and vimentin were analyzed by IF. (E) WB was performed to detect the activation of PAK, LIMK and cortactin in the skeleton signal pathway. (F) Phalloidin IF staining analyzes the pseudopodia. (G) The changes of pseudopodia were detected by SEM. (H) Transwell invasion assay was performed to analyze the invasive ability of cells.