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. 2020 Sep 2;10(24):11013–11025. doi: 10.7150/thno.47516

Figure 1.

Figure 1

TLR5 deficiency attenuates the DOX-induced cardiotoxic effects in mice. (A-B) TLR5 mRNA and protein expression in the hearts at three days after DOX injection (n = 6-8). (C) Expression of TLR5 protein in NRCMs received DOX for 24 h (n = 6). (D) Body weight change (n = 8). (E) The ratio of heart weight to tibia length (n = 8). (F-G) Serum concentration of CK-MB and LDH (n = 6). (H) H&E staining. Black line indicates the thickness of the myofibril (n = 8). (I) Ejection fraction in TLR5 KO or WT mice with or without DOX (n = 6). (J) DHE staining (n = 6). (K-L) The levels of 4-HNE and MDA in DOX-treated hearts (n = 6). (M) NADPH oxidase activity (n = 6). (N) Protein expression of NOX isoenzymes (n = 6). (O) Apoptosis measured by TUNEL staining (n = 6). *P < 0.05 versus WT Saline; #P < 0.05 versus WT DOX group.