KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-Kv4.2 | Abcam | Cat# ab16719; RRID: AB_443443 |
| Rabbit polyclonal anti-Rab4 | Cell Signaling Technology | Cat# 2167; RRID: AB_2253579 |
| Rabbit monoclonal anti-β-Amyloid | Cell Signaling Technology | Cat# 8243; RRID: AB_2797642 |
| Mouse monoclonal anti-β-Amyloid | Biolegend | Cat# 803001; RRID: AB_2564653 |
| Mouse monoclonal anti-β-Actin | Sigma-Aldrich | Cat# A5316; RRID: AB_476743 |
| HRP Goat anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat# 31460; RRID: AB_228341 |
| HRP Goat anti-Mouse IgG (H+L) | Thermo Fisher Scientific | Cat# 31430; RRID: AB_228307 |
| Biotinylated Goat anti-Rabbit IgG | Vector Laboratories | Cat# BA-1000; RRID: AB_2313606 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| (R)-(+)-carvedilol | Zhang et al., 2015 | N/A |
| D(−)-2-Amino-5-phosphonopentanoic acid | Sigma-Aldrich | Cat# A8054; CAS# 79055-68-8 |
| 6,7-dinitroquinoxaline-2,3-dione | Sigma-Aldrich | Cat# D0540; CAS# 2379-57-9 |
| Picrotoxin | TOCRIS | Cat# 1128; CAS# 124-87-8 |
| Tetrodotoxin citrate | Abcam | Cat# ab 120055; CAS# 18660-81-6 |
| Tetraethylammonium chloride | Sigma-Aldrich | Cat# T2265; CAS# 56-34-8 |
| NS5806 | Sigma-Aldrich | Cat# N2540; CAS# 426834-69-7 |
| Critical Commercial Assays | ||
| Premium Grade Sulfo NHS-SS-Biotin | Pierce | Cat# PG82077 |
| Streptavidin Agarose | Pierce | Cat# 20349 |
| FD Cresyl Violet Solution | FD NeuroTechnologies | Cat# PS102–01 |
| FD Rapid GolgiStain Kit | FD NeuroTechnologies | Cat# PK401 |
| Experimental Models: Cell Lines | ||
| Flp-In T-Rex HEK293 cell | Invitrogen | Cat# R78007; RRID: CVCL_U427 |
| RyR2 WT HEK293 cell line | Jiang et al., 2004 | N/A |
| RyR2 E4872Q HEK293 cell line | Chen et al., 2014 | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: 5xFAD+/− | The Jackson Laboratory | MMRRC Stock No: 34848-JAX |
| Mouse: RyR2 E4872Q+/− | Chen et al., 2014 | N/A |
| Mouse: Thy1-GCaMP6f+/− | The Jackson Laboratory | Stock No: 025393 |
| Recombinant DNA | ||
| Plasmid: Kv4.2 | This paper | N/A |
| Plasmid: EGFP | This paper | N/A |
| Plasmid: KChIP4 | This paper | N/A |
| Plasmid: PS1 | This paper | N/A |
| Plasmid: PS1 M146L | This paper | N/A |
| Plasmid: PS1 L286V | This paper | N/A |
| Software and Algorithms | ||
| pCLAMP 10.6 | Molecular Devices | https://www.moleculardevices.com/ |
| OriginPro 9.0 | OriginLab | https://www.originlab.com/ |
| MATLAB | MathWorks | https://www.mathworks.com/products/matlab.html |
| ScanImage 3.8.1 | Pologruto et al., 2003 | RRID: SCR_014307 |
| ImageJ | https://imagej.nih.gov/ij | |
| NeuroSeg | Guan et al., 2018 | N/A |
| RECONSTRUCT | Risher et al., 2014 | N/A |
| Graphpad Prism 7 | Graphpad Software | https://www.graphpad.com/ |