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. 2020 Oct 3;538:54–62. doi: 10.1016/j.bbrc.2020.09.131

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Fig. 2 — Biophysical characterization of the SARS-CoV-2 N CTD. (A) SDS-PAGE analysis showing the migration of the SARS-CoV-2 N CTD as a monomeric band (M, molecular weight marker). (B) SEC-MALS analysis showing elution of the SARS-CoV-2 N CTD as a dimer. (C). Summary of the homo-oligomeric in solution profile and thermal stability values for the SARS-CoV-2 N CTD. (D) Raw data, residuals of the fits and sedimentation coefficient distribution of AUC analysis showing sedimentation of the SARS-CoV-2 N CTD as a dimer. (E) Raw data (dark green) and first derivative of 330/350 nm fluorescence ratio (light green) of Nano-DSF analysis showing inflection points and Tm of the SARS-CoV-2 N CTD as two conformational states.