Fig. 3.

Downstream effects and keys players of HAS3-EVs in target cells. a Antibody array analysis showing the cell cycle protein expression of HaCaT cells, treated with HAS3-EVs compared to untreated control. The protein expression values of HaCaT cells treated with HAS3-EVs were charted based on fold change compared to untreated control. Six replicates of each protein on the array (FC fold change), b Differentially enriched canonical pathways of HaCaT cells proteome shared amid groups treated with MV3-EVs and HAS3-EVs, as compared to control. Arrow heads point to pathway activation (z-score > 2) or inhibition (z-score < -2), as predicted by IPA algorithms. c A heat map of row-wise scaled group mean mRNA expression of control HaCaT cells or cells treated with MV3- or HAS3-EVs (N = 3 per group) for genes involved in hedgehog signaling