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. 2020 Oct 3;11(10):826. doi: 10.1038/s41419-020-03028-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Epidermis and dermis from six psoriasis lesional skin and six healthy controls were separated. a EZH2 mRNA levels were detected by Real-Time PCR. b Protein levels of EZH2 and H3K27me3 were detected by Western Blotting. Band intensities were quantified relative to GAPDH. c, d Immunofluorescent analysis of EZH2 and H3K27me3 compared with normal skin. Scale bar = 100 μm. e Immunofluorescent analysis of EZH2 in HaCat cells after treatment of psoriasis-related mixed cytokines (TNF-α 50 ng/ml, IFN-γ 20 ng/ml, IL-17A 30 ng/ml, and IL-22 30 ng/ml) for 24 h. Scale bar = 50 μm. f, g HaCat cells were treated with mixed cytokines for 12, 24, and 48 h. EZH2 and H3K27me3 levels were detected by Western Blotting. Band intensities were quantified relative to GAPDH or total H3. *P < 0.05; **P < 0.01; ***P < 0.001.