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. 2020 Oct 3;11(10):826. doi: 10.1038/s41419-020-03028-1

Fig. 2. EZH2 regulated proliferation of keratinocytes.

Fig. 2

a HaCat cells were infected with lentiviral non-targeting control (Control-shRNA) or ShRNA targeting EZH2 (EZH2-shRNA1, 2, and 3). The efficiency of EZH2 and H3K27me3 knockdown was verified by Western Blotting. b HaCat cells infected with Control-shRNA or EZH2–shRNA2 were treated with mixed cytokines. EZH2 and H3K27me3 protein levels were detected by Western Blotting. Band intensities were quantified relative to β-actin or total H3. Proliferation of cells was analyzed by Cell Counting Kit-8 (c) and EdU assay (d). Scale bar = 100 μm. e Cells were subjected to lentiviral infection to overexpress EZH2. The effect of overexpressing EZH2 and H3K27me3 was detected by Western Blotting. Band intensities were quantified relative to β-actin or total H3. Proliferation of cells was analyzed by Cell Counting Kit-8 (f) and EdU assay (g). Scale bar = 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001.