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. Author manuscript; available in PMC: 2020 Oct 4.
Published in final edited form as: Cell. 2019 Aug 8;178(5):1145–1158.e20. doi: 10.1016/j.cell.2019.07.011

Figure 5. P-E interactions are largely impervious to acute removal of Mediator and PolII but dependent on cohesin.

Figure 5.

(A) Hi-C contact matrices for the Irf2bp2 locus in Mediator degron (lower left) vs. control WT cells (upper right). Irf2bp2 promoter-SE contacts are underlined in the matrices and the ratio of Hi-C signals between degron and control (1.1) are indicated. H3K27Ac, mRNA, CTCF, RAD21, MED26, PolII, and H3K4me3 ChIP-Seq tracks are shown and the SE is boxed. D = degron treatment. (B) Box plot comparing MED26, PolII, and RAD21 signals in control (empty) or Mediator degron (blue) cells. Comparison of P-E interactions as determined by enhancer proximity (left) or ChIA-PET (right) are shown. (C) Same analysis as in A for the MIR31 locus in HCT116 RAD21 degron and controls cells. Hi-C signal ratio for MIR32 and MTAP promoters are 0.45 and 0.53 respectively. (D) Same analyses as in B for HCT-116 cells. For B and D data are represented as mean +/− SEM.