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. Author manuscript; available in PMC: 2020 Oct 4.
Published in final edited form as: Cell. 2019 Aug 8;178(5):1145–1158.e20. doi: 10.1016/j.cell.2019.07.011

Figure 6. Analysis of Mediator-PolII degron.

Figure 6.

(A) RAD21degronRPB1+/− cells were created by deleting one allele of RPB1 from HCT116 RAD21degron cells to make them highly susceptible to PolII degradation by α-Amanitin. (B) RAD21degronRPB1+/− cells were untreated or treated for 3h with α-Amanitin, 6h with Auxin, or with a combination of Auxin and α-Amanitin and examined for PolII, MED26, or RAD21 occupancy. Auxin-washed cells led to RAD21 recovery in the presence of α-Amanitin (last row). (C) Bar graph showing the total number of reproducible P-P contacts (blue bars) or P-E contacts (orange bars) in RAD21degronRPB1+/− cells that were either untreated or treated with Auxin, α-Amanitin, or both. (D) Representative example of promoter contacts for TMEM67 in RAD21degronRPB1+/− cells grown under the conditions indicated. Scores in blue denote the significance of the interactions and the interacting enhancer domain is boxed.