FIGURE 2.

Effects of GIC self‐renewal and proliferation treated with I‐BET151 in vitro. (A) The representative images of GICs neurospheres showed that neurosphere formation ability of GICs was significantly inhibited by I‐BET151 treatment. The outlined sections of top images were defined as higher magnification sections below. n = 5, scale bar = 200 µm. (B) The quantification of numbers and diameter of the GICs neurospheres showing that neurosphere formation ability of GICs were obviously inhibited after I‐BET151 treatment. (C) The ability of GICs proliferation was showed by cell viability assay. (D) The ability of GICs self‐renewal was detected by in vitro limiting dilution assay. Data are shown as means ± SD, n = 5, *P < .05, **P < .01, likelihood ratio test. Data in B, C are shown as means ± SD, n = 5, *P < .05, **P < .01, ****P < .0001, Student's t‐test