FIGURE 4.

Impacts of GIC self‐renewal and proliferation treated by Notch1 inhibition in BRD4 overexpression GICs. (A) The representative images of GICs neurospheres showed that neurosphere formation ability of GICs was significantly inhibited by DAPT (10 µM) treatment and shNotch1 knockdown. The outlined sections of top images were defined as higher magnification sections below. n = 5, Scale bar = 200 µm. (B) The quantification of numbers and diameter of the GICs neurospheres showing that neurosphere formation ability of GICs were obviously inhibited after DAPT treatment and shNotch1 knockdown. (C) The ability of GICs proliferation was showed by cell viability assay. (D) The ability of GICs self‐renewal was detected by in vitro limiting dilution assay. Data are shown as means ± SD, n = 5, **P < .01, likelihood ratio test. Data in B, C are shown as means ± SD, n = 5, *P < .05, **P < .01, ***P < .001, ****P < .0001, Student's t‐test