FIGURE 3.

Transgene expression using ANAMs determined by luciferase and GFP reporter genes. A, Effects of ANAM‐β, ANAM‐2Sβ, and ANAM‐β‐κB on improving the efficiency of luciferase transgene expression in 293t and 5637 cells. B, Effects of ANAM‐κB and ANAM‐2SκB on improving the efficiency of luciferase transgene expression in 293t cells and 5637 cells. C, Secondary structure and the nucleic acid sequence of ANAM‐β‐κB. D, Effects of ANAM‐β, ANAM‐2Sβ, and ANAM‐β‐κB on improving the efficiency of GFP transgene expression in 293t cells assessed by flow cytometry. E, Effects of ANAM‐κB, and ANAM‐2SκB on improving the efficiency of GFP transgene expression in 5637 cells assessed by flow cytometry. F, Effects of ANAM‐β, ANAM‐2Sβ, and ANAM‐β‐κB on improving the efficiency of GFP transgene expression in 5637 cells assessed by flow cytometry. G, Effects of ANAM‐κB, and ANAM‐2SκB, on improving the efficiency of GFP transgene expression in 5637 cells assessed by flow cytometry. All experiments were repeated three times. ^* P < .05, ^** P < .01, ^*** P < .001 compared to the ANAM control group