Table 1. Haplogroup profiles for the ARPE-19 cybrids.

Illustration of cybrid cell lines used with their associated haplogroup type along with the age and gender of the respective donor. Cytoplasmic hybrids (cybrids), cell lines with identical nuclear genomes but different mitochondrial genomes, were created and used in the fifth passage for all experiments. Cybrid cells were created using platelets isolated from peripheral blood that was fused with Rho0 (mtDNA free) ARPE-19 cells. The mtDNA haplogroups for each subject and cybrid cell line were identified using polymerase chain reaction (PCR) along with restriction enzyme digestion and mtDNA sequencing. DNA was extracted from the individual cybrids (n = 7 for L cybrids, n = 4 for [A+B] cybrids, and n = 3 for D cybrids) using a kit. Next Generation Sequencing (NGS) technology was used to sequence both strands of mtDNA independently in both directions. This was done to quantitate the haplogroup-defining single nucleotide polymorphisms (SNPs), private SNPs (not defining haplogroups), and low-frequency heteroplasmy SNPs across the entire mitochondrial genome. All of the SNPs identified had a Quality Score of 100 and Passed all of the Filters.

Subject	Haplogroup	Age	Gender
Cyb L 11-38	L0a1a1	38	F
Cyb L 11-30	L1b1a	54	F
Cyb L 13-126	L1b1a7	39	F
Cyb L 13-124	L1b2a	31	M
Cyb L 13-125	L1c2a1	52	F
Cyb L 11-31	L2b2	42	M
Cyb L 11-17	L3e1a1a	64	F
Cyb A 11-16	A2	42	F
Cyb A 11-14	A2w	34	F
Cyb B 12-40	B	31	M
Cyb B 13-68	B2a	45	F
Cyb D 13-55	D4a2b	45	F
Cyb D 11-18	D4a6	39	M
Cyb D 11-29	D4d	37	F