Fig. 4.

(a) Phylogenetic analysis (400 bootstrap replicates) of cnidarian MCOs. Arrows point to proteins found in coral skeletons. Bold text: sequences determined from genome data, others from transcriptomes. Aligning algorithm: MAFFT. Best-fit model: WAG + G + I. Number on nodes: bootstrap support and posterior probability values. Support values in bold: node supported (>50) in MUSCLE-based phylogeny (supplementary fig. 4, Supplementary Material online). (b) Section of multiple consensus (60%) sequences alignment for the three “corallimorph + scleractinian” clades. Blue box highlights the absence of the type-I copper-binding histidine in clades 2 and 3. Histidine classification based on Takeuchi et al. (2016). All alignments available at https://gitlab.lrz.de/palmuc/Concietal_proteomics_skeletomes (last accessed August 11, 2020).