Fig. 2.

Cryo-electron tomography analyses of the cell envelopes of Tuwongella immobilis and Gemmata obscuriglobus. Tomograms of (A) T. immobilis and (B) Gem. obscuriglobus; scale bar: 250 nm. Corresponding density profiles were recorded within the highlighted area (red square) across the cell envelope (from inside to outside: IM, inner membrane; OM, outer membrane; LPS, putative lipopolysaccharide); scale bar: 250 nm, thickness: 25 voxels. (C) Boxplots of the measured IM–OM distances of T. immobilis (n = 60) and of Gem. obscuriglobus (n = 60). *Measurements for Planctopirus limnophila (n = 60) are based on previously published cryo-ET data (Boedeker et al. 2017).