Fig. 3.
Cryo-electron tomography analyses of protein complexes that span across the cell envelopes in Tuwongella immobilis and Gemmata obscuriglobus. Outer-membrane embedded protein complexes in tomograms of (A) T. immobilis and (E) Gem. obscuriglobus without visible connection to the cytoplasmic membrane. Outer membrane embedded protein complexes with clear connection to cytoplasmic membrane in tomograms of (B) T. immobilis and (D) Gem. obscuriglobus; scale bar: 100 nm, thickness: 25 voxels. Segmentation of embedded protein complexes on the cell surface (indicated by spheres) in tomographs (thickness: 1 voxel) of (C) T. immobilis and (F) Gem. obscuriglobus. Colors of spheres correspond to arrow colors in (A and B) and (D and E); scale bar: 500 nm, thickness: 1 voxel.