Figure 4. Activation of mTOR signaling pathway following PFOA exposure.
(A) Histograms demonstrating increased expression of Mtor and Kit with increased PFOA exposure, suggesting activation of mTOR pathway. (B) Histograms also quantifying mRNA levels of inhibitors of mTOR pathway namely Pten and Tsc1. A significant reduction in Pten expression was noted at higher PFOA exposure. The expression levels were normalized with GAPDH. n = 3 for all experimental data. PFOA treatment concentrations are shown as the mass in legends per kg mouse per day. Significance codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1.