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. 2020 Sep 30;13:9623–9629. doi: 10.2147/OTT.S263250

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© 2020 Makino et al.

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IL-6 and TNF-α in the bronchoalveolar lavage (BAL). The protein levels of IL-6 and TNF-α in the BAL were measured. The BAL was collected from the left lobe by washing three times with 500 μL saline through the left bronchus. The concentrations of IL-6 and TNF-α in the BAL were determined using a mouse IL-6 Quantikine ELISA kit and a mouse TNF-α ELISA Development Kit. (A) IL-6 expression in the BAL was significantly higher in the smoking group compared with the control group (31.2 ± 6.2 vs 22.1 ± 3.8, P = 0.014). (B) TNF-α expression in BAL was significantly higher in the smoking group compared with the control group (145.5 ± 46.7 vs 109.5 ± 17.5, P = 0.047).