Table 1.
Ions monitored for DON and its conjugated forms in quadrupole-orbitrap MS and the ion transitions in triple quadrupole MS
| Analyte | Theoretical exact mass | Ion (negative ion mode) | Elemental composition | Quadrupole-orbitrap (m/z) | Triple quadrupole (m/z) |
|---|---|---|---|---|---|
| DON | 296.1260 | [M-H]- | [C15H20O6-H]- | 295.1187 | |
| Fragment 1 | [C14H18O5-H]- | 265.1081 | 295.1 > 265.1 | ||
| Fragment 2 | [C14H16O4-H]- | 295.1 > 247.1 | |||
| 13C-DON | 311.1763 | [M-H]- | [C15H20O6-H]- | 310.1690 | |
| Fragment 1 | [C14H18O5-H]- | 279.1551 | 310.2 > 279.2 | ||
| DON3G | 458.1788 | [M-H]- | [C21H30O11-H]- | 457.1715 | |
| Fragment 1 | [C20H28O10-H]- | 427.1610 | 457.0 > 427.0 | ||
| Fragment 2 | [C14H16O4-H]- | 457.0 > 247.1 |