Table 2.
Results from ID-LFIA-Q-orbitrap MS analysis of wheat and beer samples
| Sample | Mean absolute intensity of peak height ± SD | LFIA screening result (mg/kg, mean ± SD) | |||
|---|---|---|---|---|---|
| DON | DON3G | ||||
| m/z | |||||
| 295.1187 | 265.1081 | 457.1715 | 427.1610 | ||
| Blank wheat | - | - | - | - | < 0.50 (± 0.00) |
| Spiked DON wheat (1) | 1045 (± 5.0) | 338 (± 8.5) | - | - | 2.63 (± 0.09) |
| Spiked DON wheat (2) | 1311 (± 29.0) | 293 (± 5.5) | - | - | 2.48 (± 0.01) |
| Spiked DON wheat (3) | 1046 (± 64.0) | 291 (± 17.5) | - | - | 2.57 (± 0.13) |
| Spiked DON wheat (4) | 1710 (± 250.0) | 473 (± 22.5) | - | - | 2.35 (± 0.00) |
| Spiked DON wheat (5) | 1665 (± 130.0) | 564 (± 3.5) | - | - | 2.54 (± 0.04) |
| Spiked DON wheat (6) | 1550 (± 135.0) | 507 (± 41) | - | - | 2.56 (± 0.11) |
| Spiked DON wheat—1-week stability of developed ID striptest | 1580 (± 50) | 994 (± 47) | - | - | 2.60 (± 0.01) |
| Incurred wheat DON | 1165 (± 45) | 287 (± 21) | - | - | 2.57 (± 0.00) |
| Spiked DON3G wheat | - | - | 2230 (± 30) | 983 (± 77) | 2.64 (± 0.00) |
| Incurred beer UCT | 3360 (± 220) | 745 (± 34) | 2900 (± 140) | 820 (± 2) | > 5.50 (± 0.00) |
Conditions: duplicate infusions of the same final ID-LFIA dissociation solution in Q-orbitrap MS. SD is the standard deviation of the duplicate measurement. The LFIA screening result was obtained with the r-Biopharm LFIA for DON and quantification with the associated smartphone application. SD is the standard deviation of duplicate reading of the same LFIA