Fig. 1.

Construction of α‐AMY knockout and complementation strains.

A. Schematic illustration of generation of the ME49Δα‐amy and Comp‐α‐AMY strains via CRISPR/CAS9‐mediated technique.

B. Diagnostic PCRs on a Δα‐amy: DHFR* and Comp‐α‐AMY clones.

C. Western blotting confirmed disruption of α‐AMY in transgenic parasites. Parasite lysates were incubated with anti‐α‐AMY antibody, ALD served as the loading control.

D. The presence of α‐AMY was confirmed by IFA. Samples were stained with mouse anti‐Ty and rabbit anti‐T. gondii ALD.