Fig. 7.

Levels of cytokines and Toxoplasma‐specific IgG in the serum of mice 30 and 75 days post‐vaccination. A–D. Levels of IFN‐γ, IL‐12 p70, TNF‐α and IL‐4 were measured by ELISA. E. Levels of Toxoplasma‐specific total IgG and IgG subclass (IgG1 and IgG2a) measured by indirect ELISA. Serum samples of naïve mice were determined as controls, and three mice from each group were analysed. The data are presented as the mean ± SEM of three mice from each group. ***P < 0.001, *P < 0.05, ‘ns’ indicated as not significant, Student’s t‐test.