Fig. 8.

Cytokines production by splenocytes of naïve mice and vaccinated mice after Toxoplasma antigen stimulation.

A. The serum samples of naïve mice and immunized mice were collected to measure the indicated cytokines 145 days post‐immunization.

B–E. The splenocytes of vaccinated mice were harvested 145 days post‐vaccination. Control is the group treated with foetal bovine serum, TSA is the group treated with total Toxoplasma antigen, and ConA is the group treated with concanavalin A as a positive control. The levels of IFN‐γ, IL‐12 p70, TNF‐α and IL‐4 were measured by ELISA. Three mice from each group were analysed, and each treatment condition was repeated three times, ***P < 0.001, **P < 0.01, *P < 0.05, Student’s t‐test.