Fig. 4.

A. cylindrica dioxygenase activity characterization.

A. Enzyme activity at different concentrations of L‐DOPA, measured in 50 mM sodium phosphate buffer pH 7.0.

B. Double‐reciprocal plot showing the inverse activity rate as a function of the inverse of substrate concentration.

C. Effect of pH on the activity of the novel dioxygenase enzyme. Reactions were performed with 2.5 mM L‐DOPA in 50 mM sodium acetate buffer for pH values ranging from 3.5 to 5.0 and in 50 mM sodium phosphate buffer for pH values ranging from 5.5 to 8.5.