Fig. 6.

Phylogenetic analysis of characterized betalamic acid‐forming DODAs and related proteobacteria sequences. The analysis was performed with the conserved block of G. diazotrophicus which includes the His 101. This residue is conserved in all characterized 4,5‐DOPA‐dioxygenases known to produce betalamic acid (green dot) and several proteobacteria. The conserved residues were also detected in the novel Anabaena cylindrica enzyme here described to produce dopaxanthin (pink dot), and it conforms an independent clade. Multiple sequence alignment was performed using Clustal Omega, and the phylogenetic tree was inferred by using the phylogeny tool from the ClustalW2 package and plotted using ITOL.